At JAXA PCG (Protein Crystal Growth) project, we've set up laboratories necessary for characterizing and improving the properties of your samples. Here's an overview of our facilities.
Area for Protein Crystallization at 20℃
This area is for protein crystallization controlled at 20℃. Various instruments used for protein crystallization are available.
Major instruments available in this area
Crystallization robot (mosquito)
Low-volume Liquid Dispensing instrument (dragonfly)
Dynamic Light Scattering (DLS) Detector instrument (Zetasizer μV ZMV2000)
Protein Crystallization Imager (Rock Imager 1000)
Low-temperature Incubator (MIR-254S-PJ)
Anaerobic Chamber (HOTK-200-W-S)
Refrigerated Benchtop Centrifuge (Allegra X-30R)
Plate Centrifuge (PlateSpin3)
Thermal Cycler (MiniAmp)
Spectrophotometer & Fluorometer (DS-11)
Semi-automated Electronic Pipetting instrument (epMotion 96)
Stereo Microscope (M205C)
Ultra Microvolume UV-Vis Spectrophotometers & Fluorometer (NanoDrop One)
DLS Detector instrument (DynaPro NanoStar)
High-speed Refrigerated Micro Centrifuge (MX-307), CFC-free Mini-size Bath (e-Cooling Bucket ECB), Precision Electronic Balance (MSA225S100DI)
Area for Protein Sample Preparations
Around ten researchers and technical staffs work here.
Major instruments available in this area
Automated Liquid Handling System (STARLet AutoLoad)
Gel documentation System (FUSION SOLO S)
Electroporation System (Gene Pulser Xcell)
Centrifugal Evaporator (CVE-2100)
Block Bath Shaker (MyBL-100CS), High-speed Micro Centrifuge (CT15RE)
High-speed Refrigerated Micro Centrifuge (MX-307)
Ultracentrifuges (CS100FNX, CP100NX)
Desiccator (AUTO DRY)
High Pressure Homogenizer (LAB2000), Autoclave (LSX-300)
Low-temperature Incubator (MIR-154S-PJ), Autoclave (HV-100ⅡLB)
Ultra-Low Temperature Freezer (MDF-DU300H-PJ)
Ductless Fume Hood (Captair 392 Smart)
Electronic Balances (TWC623N, UP6202Y)
Area for purification
Chromatography Systems used for the protein sample purification are equipped in this area.
Major instruments available in this area
Semi-automated Tangential Flow Filtration/Cross Flow Filtration System (AKTA flux S)
Ultrapure Water Production Equipment (Milli-Q)
Bio Clean Bench (AirStreamPro ESC-BCB-4E7)
Bioshaker (BR-3000LF)
Cool Incubator (KMH-050)
Chromatography System (AKTA avant25)
High-speed Centrifuge (Avanti JXN-26)
Bioshaker (BR-23FP MR)
Area for X-ray diffraction
Instruments such as an X-ray diffraction system, computers for the analyses, crystal mounting devices and Lab bench are equipped in this area.
Major instruments available in this area
X-ray Diffraction System (XtaLAB MM003)
Solution-free Crystal Mounting Device Container (GOT2000)
Area for low-temperature crystallization
This area is a cabin-type Lab at 4°C for handling protein samples crystallized at 4°C. Instruments such as crystallization imager and UV fluorescence Imager are equipped inside.
Major instruments available in this area
Protein Crystallization Imager (Rock Imager 54)
Automated UV fluorescence Imager (UVEX-p)
Chromatography System (AKTA start)
Stereo Microscope (SZX-7-TR-30-2-SET)
Ultrasonic Homogenizer (SONICSTAR85)
Lab for cultivation
This area is for protein production and purification using insect cells and mammalian cells.
Major instruments available in this area
Gel Permeation Chromatography (GPC) / Size Exclusion Chromatography (SEC) System (OmniSEC resolve)
8-capillary Sequencing Instrument (Generic Analyzer 3500)
Fluorescent Liquid Chromatography System (Prominence)
Incubator Shaker (Innova 42)
Ultrapure Water Production Equipment (Milli-Q)
Real-Time PCR System (StepOnePlus)
Ultracentrifuge (CP 80NX), Autoclave (LSX-500)
Biosafety Cabinet (AC2-4N7)
Culture Microscope (CKX53)
Microplate Reader (VarioskanLUX)
Real-Time PCR System (Rotor-Gene Q)
CO2 Incubator (MCO-170AICUVD)
Chromatography System (AKTA go)
Chromatography System (AKTA pure 25)
Chromatography System (AKTA pure 25)
Chromatography System (AKTA start)
